Microbial community changes in TNT spiked soil bioremediation trial using biostimulation, phytoremediation and bioaugmentation

    Hiie Nõlvak Info
    Jaak Truu Info
    Baiba Limane Info
    Marika Truu Info
    Guntis Cepurnieks Info
    Vadims Bartkevičs Info
    Jaanis Juhanson Info
    Olga Muter Info

Abstract

Trinitrotoluene (TNT), a commonly used explosive for military and industrial applications, can cause serious environmental pollution. 28-day laboratory pot experiment was carried out applying bioaugmentation using laboratory selected bacterial strains as inoculum, biostimulation with molasses and cabbage leaf extract, and phytoremediation using rye and blue fenugreek to study the effect of these treatments on TNT removal and changes in soil microbial community responsible for contaminant degradation. Chemical analyses revealed significant decreases in TNT concentrations, including reduction of some of the TNT to its amino derivates during the 28-day tests. The combination of bioaugmentation-biostimulation approach coupled with rye cultivation had the most profound effect on TNT degradation. Although plants enhanced the total microbial community abundance, blue fenugreek cultivation did not significantly affect the TNT degradation rate. The results from molecular analyses suggested the survival and elevation of the introduced bacterial strains throughout the experiment.

First published online: 15 Feb 2013

Keywords:

TNT, bioaugmentation, biostimulation, phytoremediation, microbial community

How to Cite

Nõlvak, H., Truu, J., Limane, B., Truu, M., Cepurnieks, G., Bartkevičs, V., Juhanson, J., & Muter, O. (2013). Microbial community changes in TNT spiked soil bioremediation trial using biostimulation, phytoremediation and bioaugmentation. Journal of Environmental Engineering and Landscape Management, 21(3), 153-162. https://doi.org/10.3846/16486897.2012.721784

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October 14, 2013
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2013-10-14

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How to Cite

Nõlvak, H., Truu, J., Limane, B., Truu, M., Cepurnieks, G., Bartkevičs, V., Juhanson, J., & Muter, O. (2013). Microbial community changes in TNT spiked soil bioremediation trial using biostimulation, phytoremediation and bioaugmentation. Journal of Environmental Engineering and Landscape Management, 21(3), 153-162. https://doi.org/10.3846/16486897.2012.721784

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